Methods for determining modulators of insect transient receptor potential V (TRPV) channel
OwnerAlexandre Nesterov, Ramani Kandasamy +2inventors
InventorsAlexandre Nesterov, Ramani Kandasamy, Barbara Wedel, Vincent L Salgado
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Abstract
The present invention relates to a screening method for determining whether or not a candidate compound is a modulator of an insect transient receptor potential V (TRPV) channel. The present invention further provides a method of insect control by applying to an insect-specific TRPV channel modulator determined by the screening method. The present invention further relates to an expression vector that includes a nucleic acid molecule coding for an insect TRPV channel. Also, the present invention relates to cell that includes the expression vector encoding a TRPV channel.
Claims12
A method for determining whether or not a candidate compound is a modulator of an insect transient receptor potential V (TRPV) channel, comprising:
- (a) providing a first cell expressing an insect TRPV channel, wherein the insect TRPV channel co-expresses insect Nanchung and Inactive proteins:
- (b) contacting the first cell with a candidate compound;
- and
- (c) assaying for a modulation of the insect TRPV channel, wherein the modulation identifies the candidate compound as the modulator of the insect TRPV channel,
- wherein the assaying step comprises at least one of the following steps:
- (1) detecting calcium ion mobilization in the first cell in response to the candidate compound;
- or
- (2) detecting a membrane potential in the first cell in response to the candidate compound;
- or
- (3) comparing calcium ion mobilization in the first cell in the absence of the candidate compound with calcium ion mobilization in the first cell in the presence of the candidate compound;
- or
- (4) comparing a membrane potential in the first cell in the absence of the candidate compound with a membrane potential in the first cell in the presence of the candidate compound;
- (5) comparing calcium ion mobilization in the first cell in the presence of the candidate compound with calcium ion mobilization reference level indicative of no modulation of the TPRV channel;
- or
- (6) comparing a membrane potential in the first cell in the presence of the candidate compound with a membrane potential reference level indicative of no modulation of the TPRV channel.
Dependencies are inferred from the claim text.