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US 10,024,780 B2Granted
Methods for detecting events in a flow cytometer
AssigneeAbbott Laboratories (Diagnostics Division)
InventorsAmish P. Shah, Frederic M. Hulett, III
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Abstract
Aspects of the present disclosure include methods for detecting events in a flow cytometer. Also provided are methods of detecting cells in a flow cytometer. Other aspects of the present disclosure include methods for determining a level of contamination in a flow cell. Computer-readable media and systems, e.g., for practicing the methods summarized above, are also provided.
Claims14
A method for detecting events in a flow cytometer, comprising:
- flowing particles through a flow cell of the flow cytometer;
- optically interrogating the particles flowing through the flow cell;
- extracting putative event features;
- time-stamping putative events;
- determining a time difference between a putative previous event and a putative current event;
- comparing the time difference to a threshold duration, wherein:
- if the time difference is greater than the threshold duration, storing the putative current event as a current event;
- and
- if the time difference is less than the threshold duration, comparing a peak height feature of the putative current event and a peak height feature of the putative previous event to a threshold peak height, wherein:
- if the peak height feature of the putative current event is less than the threshold peak height, discarding the putative current event;
- and
- if the peak height feature of the putative previous event is greater than the threshold peak height, storing the putative previous event as a previous event.
9 more elements
Dependencies are inferred from the claim text.