High resolution melting analysis as a prescreening tool
AssigneeRoche Molecular Systems, Inc.
InventorsReinhard Buettner, Stefanie Froehner, Sabine Merkelbach-Bruse, Jasmin Ney, Angelika Roesler
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Abstract
Compositions and methods for determining an increased likelihood of a response to a targeted treatment of a cancer disease including isolating genomic DNA from a patient sample, amplifying a fragment of DNA by means of PCR with a specific pair of amplification primers, determining if the amplified fragment comprises a wildtype sequence or a mutation by means of a High Resolution Melting Analysis (HRM), and correlating the presence or absence of a mutation with an increased likelihood of success of said targeted treatment. Respective primer pairs, compositions and kits are also claimed.
Claims10
A method of treating a cancer patient comprising:
- i) isolating genomic DNA from a sample from the cancer patient;
- ii) amplifying a fragment of said DNA by means of PCR with a specific pair of amplification primers, thereby forming an amplified fragment;
- iii) determining that said amplified fragment does not comprise a G13_V14A or G60D mutation in the KRAS gene by means of a High Resolution Melting Analysis (HRM) and DNA sequencing;
- and
- iv) treating the cancer patient with an anti-EGFR targeted treatment.
Dependencies are inferred from the claim text.