Multiple tagging of individual long DNA fragments

A method of sequencing a target nucleic acid without the use of nanodrops comprising:

• (a) combining in a single mixture (i) first fragments of the target nucleic acid, and (ii) a population of beads, wherein each bead comprises oligonucleotides immobilized thereon, said oligonucleotides comprising a tag-containing sequence, wherein each tag-containing sequence comprises a tag sequence, wherein the oligonucleotides immobilized on the same individual bead comprise the same tag-containing sequence and a majority of beads have different tag sequences;
• (b) introducing into a plurality of the first fragments multiple copies of a tag sequence, wherein the multiple copies are from a single bead per first fragment;
• (c) producing a plurality of subfragments from the plurality of first fragments, wherein a plurality of the subfragments comprise one or more tags;
• (d) sequencing the plurality of subfragments to produce a plurality of sequence reads.