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US 10,018,636 B2Granted
Method for diagnosing fibromyalgia
AssigneeEpicgenetics, Inc.
InventorBruce Gillis
Life of this patent
Estimating the term…
Abstract
The invention provides methods, kits and reagents for diagnosing fibromyalgia (FM) in an individual by determining whether the levels of one or more cytokines in the individual are altered, as compared to control levels. The altered level(s) or patterns of expression of the cytokines measured in the affected individual compared to the level from the control is predictive/indicative of FM in the individual.
Claims20
A method for detecting expression levels of cytokines in an individual previously diagnosed with fibromyalgia, comprising:
- (a) stimulating a peripheral blood mononuclear cell (PBMC) sample obtained from the individual with one or more mitogens;
- and (b) determining the expression levels in the PBMC sample of at least four cytokines selected from the group consisting of interleukin 5 (IL5), interleukin 6 (IL6), interleukin 8 (IL8), interleukin10 (IL 10), interferon-γ (IFN-γ), monocyte chemotactic protein 1 (MCP-1), macrophage inflammatory protein-1α (MIP-1α) and macrophage inflammatory protein-1β (MIP-1β) at the protein level by an antibody bead-based capture assay, wherein the bead-based capture assay comprises beads derivatized with antibodies specific to the at least four cytokines.
Dependencies are inferred from the claim text.