Stabilizied bioactive peptides and methods of identification, synthesis and use
AssigneePeptide BioSciences, Inc.
InventorElliot Altman
Life of this patent
Estimating the term…
Abstract
An intracellular selection system allows screening for peptide bioactivity and stability. Randomized recombinant peptides are screened for bioactivity in a tightly regulated expression system, preferably derived from the wild-type lac operon. Bioactive peptides thus identified are inherently protease- and peptidase-resistant. Also provided are bioactive peptides stabilized by a stabilizing group at the N-terminus, the C-terminus, or both. The stabilizing group can be a small stable protein, such as the Rop protein, glutathione sulfotransferase, thioredoxin, maltose binding protein, or glutathione reductase, an α-helical moiety, or one or more proline residues.
Claims8
A plurality of vectors, wherein each said vector comprises a nucleic acid sequence encoding a polypeptide, said polypeptide comprising a randomized peptide with a first stabilizing group covalently linked to the N-terminus of said randomized peptide, wherein said first stabilizing group lacks the capacity to participate in the formation of an intramolecular disulfide bond within the peptide, wherein said first stabilizing group is Xaa-Pro- or Xaa-Pro-Pro-, wherein Xaa is Ala and wherein said plurality of vectors comprises at least two different vectors, each of said at least two vectors encoding a different peptide.
Dependencies are inferred from the claim text.