Gene transfer into airway epithelial stem cell by using lentiviral vector pseudotyped with RNA virus or DNA virus spike protein

A method of treating cystic fibrosis, the method comprising directly contacting airway epithelial tissue of an individual having cystic fibrosis with a recombinant simian immunodeficiency virus (SIV) vector comprising a cystic fibrosis transmembrane conductance regulator (CFTR) gene operably linked to a promoter, the vector pseudotyped with spike proteins of Sendai virus envelope glycoproteins F and HN, wherein the vector infects and integrates the CFTR gene into an airway epithelial stem cell genome of the airway tissue,

• wherein the recombinant SIV vector comprises a replication-incompetent SIV genome from which 40% to 80.6% of the original SIV genome has been removed;
• wherein the airway tissue is not pretreated or pre-conditioned prior to the contacting with the vector, and
• wherein the integrated CFTR gene is expressed in the airway epithelial stem cell for at least 160 days thereby providing functional CFTR to treat the cystic fibrosis.