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US 10,017,769 B2Granted
MGMT-based method for obtaining high yeilds of recombinant protein expression
AssigneeInstitut Pasteur
InventorsPhilippe Despres, Sylvie Paulous, Elodie Crublet
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Abstract
The present invention relates to a novel enhancer of protein production in host cells. It discloses a vector for expressing recombinant proteins in these cells, comprising a nucleotide sequence encoding a) a secretion peptidic signal, b) a 6-methylguanine-DNA-methyltransferase enzyme (MGMT, EC 2.1.1.63), a mutant or a catalytic domain thereof, and c) a recombinant protein. Said MGMT enzyme is preferably the so-called SNAP protein.
Claims21
An immunoassay method for the detection of antibodies comprising:
- a) providing a recombinant protein comprising from N-terminus to C-terminus:
- a peptidic secretion signal,
- a 6-methylguanine-DNA-methyltransferase enzyme (MGMT) or mutant thereof, wherein said MGMT enzyme or MGMT mutant is the protein of SEQ ID NO:2, or a homologous sequence thereof that is at least 80% identical to SEQ ID NO:2 or at least 75% identical to SEQ ID NO:4, and
- a protein of a microorganism;
- b) contacting the recombinant protein with a biological sample containing antibodies;
- and
- c) detecting whether antibodies in the biological sample bind to the recombinant protein.
4 more elements
Dependencies are inferred from the claim text.