Methods of expanding and assessing B cells and using expanded B cells to treat disease
AssigneeDuke University
InventorsThomas F. Tedder, Ayumi Yoshizaki, Tomomitsu Miyagaki, Evgueni Kountikov, Jonathan C. Poe
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Abstract
Provided herein are methods of expanding B cells, and in particularly B10 cells capable of producing IL-10, ex vivo. The methods include incubation of harvested B cells in the presence of IL-21. Compositions comprising the ex vivo expanded B cells and methods of using the expanded B cell-containing compositions to treat diseases or conditions are also provided. Methods of assessing B10 cell function in a subject are also provided.
Claims18
A method of expanding B10 cells capable of producing IL-10 ex vivo comprising culturing polyclonal B cells harvested from a subject with IL-21 and feeder cells expressing a CD40 agonist and a B cell survival promoter and isolating the polyclonal B10 cells.
A method of expanding B cells ex vivo comprising:
- a) Culturing polyclonal B cells harvested from a subject on feeder cells expressing a CD40 agonist and a B cell survival promoter in the presence of IL-4;
- b) Culturing the B cells from step (a) on feeder cells expressing a CD40 agonist and a B cell survival promoter in the presence of IL-21;
- c) Isolating the B cells from step (b);
- and
- d) Isolating the polyclonal B10 cells from the B cells of step (c).
Dependencies are inferred from the claim text.