Fatty acid productivity
AssigneeWilliam Marsh Rice University
InventorsKa-Yiu San, Wei Li
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Abstract
The present disclosure relates to an engineered microbe capable of improved productivity of fatty acid or fatty acid derivative. An NAD+-dependent 3-oxoacyl-ACP reductase or NAD+-dependent 3-oxoacyl-CoA reductase replaces or supplements the native NADP+-dependent 3-oxoacyl-ACP reductase so as to utilize the higher availability of NAD+ rather than NADP+ in the cell. Higher production, yield and titer of fatty acids are therefore obtained. Such microbes can be combined with other mutations to further improve yield of fatty acids or fatty acid derivatives.
Claims7
An engineered FASII bacteria with improved productivity of a fatty acid or fatty acid derivative, said FASII bacteria comprising type II fatty acid synthesis enzymes (FASII) and
- a) an overexpressed NAD+-dependent 3-oxoacyl-ACP reductase or an overexpressed NAD+-dependent 3-oxoacyl-CoA reductase that replaces or supplements a native NADP+-dependent 3-oxoacyl-ACP reductase or 3-oxoacyl-CoA reductase (respectively);
- and b) one or more overexpressed acyl-ACP thioesterases (TE)
- wherein said NAD+-dependent 3-oxoacyl-ACP reductase has the amino acid sequence of SEQ ID NO. 2.
Dependencies are inferred from the claim text.