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US 10,011,849 B1Granted
Nucleic acid-guided nucleases
AssigneeInscripta, Inc.
InventorsRyan T. Gill, Andrew Garst, Tanya Elizabeth Warnecke Lipscomb
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Abstract
Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.
Claims6
A nucleic acid-guided nuclease system comprising:
- (a) a nucleic acid having at least 95% identity to SEQ ID NO:
- 22, SEQ ID NO:
- 42, SEQ ID NO:
- 128, or SEQ ID NO:
- 148, wherein the nucleic acid encodes a nucleic acid-guided nucleases comprising the amino acid sequence of SEQ ID NO:2;
- (b) an engineered guide nucleic acid capable of complexing with the nucleic acid-guided nuclease, wherein the engineered guide nucleic acid comprises any one of SEQ ID NO:
- 88, SEQ ID NO:
- 93, and SEQ ID NO:
- 94 and
- (c) an editing sequence having a change in sequence relative to the sequence of a target region in a genome of a cell and a mutation in a protospacer adjacent motif (PAM);
- wherein the system results in a genome edit in the target region in the genome of the cell facilitated by the nuclease, the engineered guide nucleic acid, and the editing sequence.
8 more elements
Dependencies are inferred from the claim text.