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US 10,011,838 B2Granted
Yeast strain and microbial method for production of pentacyclic triterpenes and/or triterpenoids
OwnerChristine Lang, Anna Lewandowskiinventors
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Abstract
The invention relates to a yeast strain and to a method for microbial production of pentacyclic triterpenes and/or triterpenoids in yeast. More particularly, the invention relates to a modified yeast strain for production of pentacyclic triterpenoids comprising at least one copy of a gene for encoding an oxidosqualene cyclase, at least one copy of a gene for encoding an NADPH-cytochrome P450 reductase and/or at least one copy of a gene for encoding a cytochrome P450 monooxygenase.
Claims36
A modified yeast strain for production of pentacyclic triterpenoids, comprising:
- i. at least one copy of a gene for encoding an oxidosqualene cyclase,
- wherein the oxidosqualene cyclase has an amino acid sequence with at least 95% sequence identity to SEQ ID NO:
- 55;
- ii. at least one copy of a gene for encoding a NADPH-cytochrome P450 reductase,
- wherein the NADPH-cytochrome P450 reductase has an amino acid sequence with at least 95% sequence identity to SEQ ID NO:
- 66, 68 or 69;
- and
- iii. at least one copy of a gene for encoding a cytochrome P450 monooxygenase,
- wherein the cytochrome P450 monooxygenase has an amino acid sequence with at least 95% sequence identity to SEQ ID NO:
- 83, 84 or 88.
7 more elements
Dependencies are inferred from the claim text.