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US 10,011,819 B2Granted
Culture medium for preparing neural stem cells and use thereof
AssigneeGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
InventorsGuangjin Pan, Duanqing Pei, Lihui Wang, Linli Wang, Yanting Xue
Life of this patent
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Abstract
Provided are a culture medium for preparing neural stem cell and use thereof, the culture medium for preparing neural stem cell comprising: a basic culture medium suitable for the growth of stem cell, and a cell signal pathway inhibitor selected from at least one of GSK inhibitor, MEK inhibitor, TGF-β inhibitor, ROCK inhibitor and BMP inhibitor.
Claims5
A medium for preparing neural stem cells from urine exfoliative cells consisting of:
- a basic medium for culturing a stem cell, and
- an inhibitor;
- consisting of glycogen synthase kinase (GSK) inhibitor, a mitogen-activated extracellular signal-regulated kinase (MEK) inhibitor, a transforming growth factor-β (TGF-β inhibitor, a Rho associated kinase (ROCK) inhibitor and a bone morphogenetic protein (BMP) inhibitor.
Dependencies are inferred from the claim text.