Thermally modulated variable restrictor for normalization of dynamic split ratios

A chromatography system comprising:

• a separation column separating a sample carried by a compressible mobile phase flow into analytes;
• a fluidic splitter in fluidic communication with the separation column to receive and divide the compressible mobile phase flow containing the separated sample into first and second mobile phase streams in accordance with a split ratio;
• a thermally modulated variable restrictor coupled between the fluidic splitter and a detector, the thermally modulated variable restrictor receiving the first mobile phase stream from the fluidic splitter, the thermally modulated variable restrictor having a temperature element in thermal communication with the first mobile phase stream to exchange heat therewith;
• and
• a controller in communication with the thermally modulated variable restrictor, the controller dynamically adjusting a temperature setting of the temperature element of the thermally modulated variable restrictor to adjust the heat exchange between the thermally modulated variable restrictor and the first mobile phase stream in order to keep the split ratio constant throughout a chromatographic run.