Electrochemical deblocking solution for electrochemical oligomer synthesis on an electrode array
AssigneeCustomArray, Inc.
InventorsKarl Maurer, John J. Cooper, Jr.
Life of this patent
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Abstract
There is disclosed an electrochemical deblocking solution for use on an electrode microarray. There is further disclosed a method for electrochemical synthesis on an electrode array using the electrochemical deblocking solution. The solution and method are for removing acid-labile protecting groups for synthesis of oligonucleotides, peptides, small molecules, or polymers on a microarray of electrodes while substantially improving isolation of deblocking to active electrodes. The method comprises applying a voltage or a current to at least one electrode of an array of electrodes. The array of electrodes is covered by the electrochemical deblocking solution.
Claims20
An electrochemical deblocking solution comprising:
- an organic base including a lutidine, a benzoquinone and a hydroquinone;
- tetraethylammonium p-toluenesulfonate as an organic salt;
- and
- a solvent selected from the group consisting of an alcohol, acetonitrile and dichloromethane.
A method of electrochemical deblocking an oligomer comprising the steps of:
- covering an array of electrodes with a deblocking solution including:
- lutidine, benzoquinone, hydroquinone and tetraethylammonium p-toluenesulfonate;
- and
- one or more solvents selected from the group consisting of an alcohol, acetonitrile and dichloromethane;
- and
- applying one or both a voltage and a current to at least one electrode of the array of electrodes.
A kit for electrochemical deblocking comprising:
- an array of electrodes adapted to connect with one or both a voltage and a current source to apply one or both a voltage and a current to at least one electrode of the array of electrodes;
- a deblocking solution including:
- lutidine, benzoquinone, hydroquinone and tetraethylammonium p-toluenesulfonate;
- and
- one or more solvents selected from the group consisting of an alcohol, acetonitrile and dichloromethane;
- and
- instructions for one or both preparing the deblocking solution and applying one or both a voltage and a current to at least one electrode of the array of electrodes.
Dependencies are inferred from the claim text.