Fucosylation-deficient cells
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsGang Chen, Darya Burakov, James P. Fandl
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Abstract
An isolated nucleic acid encoding an FX protein having a serine at position 79, a lysine at position 90, a leucine at position 136, an arginine at position 211, a serine at position 289, and a combination thereof is provided. Cells having a gene encoding a modified FX protein are provided, wherein the cells exhibit a reduced ability to fucosylate a glycoprotein at a first temperature, but exhibit the ability to fucosylate the glycoprotein at a second temperature. Methods and compositions for making glycoproteins with reduced fucosylation are provided.
Claims20
A cell that comprises a nucleic acid encoding an FX protein, wherein the FX protein comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 1, and wherein the FX protein comprises a modification relative to SEQ ID NO:
- 1, the modification comprising a substitution with a serine at position 289.
A cell that comprises a nucleic acid encoding an FX protein, wherein the FX protein comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 1, and wherein the FX protein comprises a modification relative to SEQ ID NO:
- 1, the modification comprising a substitution with a Serine at position 289 and a substitution with a lysine at position 90.
Dependencies are inferred from the claim text.