Recombinant host cells comprising phosphoketalase
AssigneeButamax Advanced Biofuels LLC
InventorsMichael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Abstract
The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
Claims6
A method for the production of isobutanol comprising growing a recombinant host cell comprising:
- (i) at least one genetic modification in an endogenous gene encoding a polypeptide having pyruvate decarboxylase activity, wherein the at least one genetic modification reduces or eliminates pyruvate decarboxylase activity;
- (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity, wherein the heterologous polynucleotide encoding a polypeptide having phosphoketolase activity is xpk1 from Lactobacillus plantarum, xpkA from Lactobacillus pentosus MD363, or 6-phosphate phosphoketolase from B. lactis;
- (iii) at least one genetic modification in an endogenous gene encoding a polypeptide having glycerol-3-phosphate dehydrogenase activity, wherein the at least one genetic modification reduces or eliminates glycerol-3-phosphate dehydrogenase activity;
- and
- (iv) an isobutanol biosynthetic pathway comprising the substrate to product conversions:
- (a) pyruvate to acetolactate,
- (b) acetolactate to 2,3-dihydroxyisovalerate,
- (c) 2,3-dihydroxyisovalerate to 2-ketoisovalerate,
- (d) 2-ketoisovalerate to isobutyraldehyde,
- (e) isobutyraldehyde to isobutanol;
- under conditions wherein isobutanol is produced and optionally recovering isobutanol.
Dependencies are inferred from the claim text.