Hairpin mRNA elements and methods for the regulation of protein translation
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsChen Ling, Arun Srivastava
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Abstract
The present invention provides novel mRNA elements capable of forming hairpin, double-stranded RNA structures independent of other non-coding RNAs. These mRNAs are stably expressed, lack polyadenylation tails, and allow minimal protein translation except when in the presence of specific proteins. Also provided, are compositions and kits comprising the mRNA element, as well as methods for its use in the regulation of protein translation. Advantageously, the disclosed elements represent a novel tool useful in regulating the expression of a wide variety of proteins of interest.
Claims23
A recombinant adeno-associated viral (rAAV) vector comprising at least a first expression cassette that comprises a promoter operably linked to at least a first nucleic acid segment that encodes at least a first mammalian polypeptide, wherein the first expression cassette lacks a polyA signal sequence, and wherein the mRNA produced from the vector lacks a polyA sequence at its 3′-end, but includes an inverted terminal repeat (ITR) at its 3′-end.
Dependencies are inferred from the claim text.