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US 10,001,476 B2Granted
Capture ligand controls, blocking probes, masking probes and methods of using the same
AssigneeGenMark Diagnostics, Inc.
InventorsMichael Leonard Wood, Aviva Ruth Jacobs
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Abstract
The invention, depending on aspect and embodiment, relates to capture probe controls, and capture and signal probe configurations and combinations of configurations that can facilitate accurate and efficient multiplex analyte detection, especially in electrochemical detection schemes.
Claims24
A method for detecting an analyte, comprising:
- providing a solid support comprising an immobilized first capture binding ligand and a dummy binding ligand comprising a second detectable label;
- contacting said immobilized first capture binding ligand with a sample suspected of containing an analyte;
- and
- analyzing said solid support for the presence of a first detectable label, wherein said solid support comprises an electrode.
1 more element
A composition for electrochemically detecting one or more analytes, comprising:
- a solid support comprising a plurality of electrodes comprising:
- an immobilized first capture binding ligand bound to an analyte and a first detectable label and a dummy binding ligand comprising a second detectable label.
A method for detecting a target nucleic acid comprising:
- a) providing a solid support comprising a plurality of electrodes, comprising:
- i) an immobilized first capture nucleic acid;
- and
- ii) a dummy capture nucleic acid;
- b) contacting said a plurality of electrodes with:
- i) a target nucleic acid;
- ii) a first signal probe with a first detectable label;
- and
- iii) a second signal probe with a second detectable label, wherein said second signal probe hybridizes to said dummy capture nucleic acid;
- c) detecting the presence or absence of said first detectable label to detect the presence or absence of said target nucleic acid and detecting the presence of said second detectable label to confirm the electrode is operational.
7 more elements
Dependencies are inferred from the claim text.