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US 10,000,758 B2Granted
Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, for producing methacrylic acid
AssigneeGenomatica, Inc.
InventorsAnthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
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Abstract
Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.
Claims23
A non-naturally occurring microbial organism comprising:
- (A) a methanol metabolic pathway, wherein said non-naturally occurring microbial organism comprises at least one exogenous nucleic acid encoding a methanol metabolic pathway enzyme expressed in a sufficient amount to enhance the availability of reducing equivalents in the presence of methanol or metabolize methanol as a carbon source for biosynthesis of methacrylate, wherein said methanol metabolic pathway comprises:
- (i) a methanol methyltransferase and a methylenetetrahydrofolate reductase;
- (ii) a methanol dehydrogenase;
- or
- (iii) a methanol dehydrogenase and a formaldehyde activating enzyme;
- and
- (B) a methacrylate (MAA) pathway.
4 more elements
Dependencies are inferred from the claim text.