Engineered enzyme having acetoacetyl-CoA hydrolase activity, microorganisms comprising same, and methods of using same
AssigneeBraskem S.A.
InventorsAvram Michael Slovic, Iuri Estrada Gouvea, Daniel Johannes Koch, Felipe Galzerani
Life of this patent
Estimating the term…
Abstract
The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10× higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.
Claims11
A modified microorganism comprising one or more polynucleotides encoding one or more enzymes in a pathway with acetoacetate as an intermediate or end-product, and an engineered enzyme having acetoacetyl-CoA substrate specificity and acetoacetyl-CoA specific hydrolase activity;
- and wherein the engineered enzyme comprises the amino acid sequence as set forth in SEQ ID NO:
- 2, SEQ ID NO:
- 4, or SEQ ID NO:
- 6.
Dependencies are inferred from the claim text.