2-deoxy-scyllo-inosose reductase
AssigneeAsahi Kasei Chemicals Corporation
InventorsKazunobu Konishi, Nobuya Itoh, Junji Kurokawa
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Abstract
The disclosure provides 2-deoxy-scyllo-inosose reductases derived from a microorganism having the ability to utilize (−)-vibo-quercitol, recombinant vectors and transformants comprising genes encoding the same, and methods of use thereof.
Claims16
A method of producing (−)-vibo-quercitol, the method comprising
- contacting 2-deoxy-scyllo-inosose with an isolated enzyme comprising a protein of (a) or (b):
- (a) a protein comprising the amino acid sequence of SEQ ID NO:
- 2, 4, 6 or 8;
- or
- (b) a protein comprising an amino acid sequence having 85% or greater identity to the amino acid sequence of SEQ ID NO:
- 2, 4, 6 or 8 and having 2-deoxy-scyllo-inosose reductase activity, and
- reacting under conditions of pH 5.0-10.0.
A recombinant vector for (−)-vibo-quercitol conversion including a gene encoding a protein of (a) or (b) below:
- (a) a protein comprising the amino acid sequence of SEQ ID NO:
- 2;
- or
- (b) a protein comprising an amino acid sequence having 85% or greater identity to the amino acid sequence of SEQ ID NO:
- 2 and having 2-deoxy-scyllo-inosose reductase activity.
A transformant for (−)-vibo-quercitol conversion comprising a gene encoding a protein of (a) or (b) below:
- (a) a protein comprising the amino acid sequence of SEQ ID NO:
- 2;
- or
- (b) a protein comprising an amino acid sequence having 85% or greater identity to the amino add sequence of SEQ ID NO:
- 2 and having 2-deoxy-scyllo-inosose reductase activity.
A recombinant vector for (−)-vibo-quercitol conversion comprising a nucleotide sequence of (a) or (b) below:
- (a) the nucleotide sequence of SEQ ID NO:
- 1;
- or
- (b) a nucleotide sequence (i) comprising a nucleotide sequence having 85% or greater identity to the nucleotide sequence of SEQ ID NO:
- 1, (ii) hybridizing under stringent conditions with DNA comprising a sequence complementary to the nucleotide sequence of SEQ ID NO:
- 1, and (iii) encoding a protein having 2-deoxy-scyllo-inosose reductase activity,
- wherein said stringent conditions comprises hybridizing at a constant temperature of 65° C. for 8-16 hours in a solution containing 6×SSC (composition of 1×SSC:
- 0.15 M sodium chloride, 0.015 M sodium citrate, pH 7.0 ), 0.5% SDS, 5× Denhardt's solution, and 100 mg/mL of herring sperm DNA, and washing by 2×SSC and 0.1% SDS at 68° C.
A transformant for (−)-vibo-quercitol conversion comprising a nucleotide sequence of (a) or (b) below:
- (a) the nucleotide sequence of SEQ ID NO:
- 1;
- or
- (b) a nucleotide sequence (i) comprising a nucleotide sequence having 85% or greater identity to the nucleotide sequence of SEQ ID NO:
- 1, (ii) hybridizing under stringent conditions with DNA comprising a sequence complementary to the nucleotide sequence of SEQ ID NO:
- 1, and (iii) encoding a protein having 2-deoxy-scyllo-inosose reductase activity,
- wherein said stringent conditions comprises hybridizing at a constant temperature of 65° C. for 8 -16 hours in a solution containing 6×SSC (composition of 1×SSC:
- 0.15 M sodium chloride, 0.015 M sodium citrate, pH 7.0), 0.5% SDS, 5× Denhardt's solution, and 100 mg/mL of herring sperm DNA, and washing by 2×SSC and 0.1% SDS at 68° C.
Dependencies are inferred from the claim text.