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US 10,000,742 B2Granted
Device and method of collection for RNA viruses
AssigneeGeneral Electric Company
InventorsErik Leeming Kvam, Robert Scott Duthie, John Richard Nelson
Life of this patent
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Abstract
The present disclosure generally relates to a method and device for inactivation and dry storage, under ambient conditions, of a biological sample containing RNA virus. Methods for collecting and recovering RNA from a biological sample and subsequent analysis for a virus are also provided.
Claims24
A method of inactivation and dry storage of a biological sample containing an RNA virus, the method comprising:
- providing a non-dissolvable, dry, solid matrix incorporated with a composition, the composition comprising at least one protein denaturant, at least one acid or acid-titrated buffer reagent impregnated therein in a dry state to provide an acidic pH on hydration;
- contacting the biological sample with the non-dissolvable, dry, solid matrix to effectively lyse cells of the biological sample to extract RNA from the lysed cells and preserve the extracted RNA in an intact state;
- drying the biological sample on the solid matrix;
- and
- storing the biological sample on the solid matrix in a dry state under ambient conditions,
- wherein the biological sample is rendered non-infectious within 30 minutes of being subjected to the drying step, and wherein the acidic pH is in a range from 2 to 7.
3 more elements
A method for testing a biological sample for presence of an RNA virus, the method comprising:
- providing a non-dissolvable, dry, solid matrix incorporated with a composition, the composition comprising at least one protein denaturant, at least one acid or acid-titrated buffer reagent impregnated therein in a dry state to provide an acidic pH on hydration;
- applying the biological sample comprising RNA to the non-dissolvable, dry, solid matrix;
- drying the biological sample on the non-dissolvable, dry, solid matrix to preserve the RNA in an intact state;
- recovering the RNA of the biological sample from the non-dissolvable, dry, solid matrix;
- and
- analyzing the recovered RNA for the presence of a viral RNA to determine the presence of the RNA virus,
- wherein the biological sample is rendered non-infectious within 30 minutes of being subjected to the drying step, and wherein the acidic pH is in a range from 2 to 7.
4 more elements
Dependencies are inferred from the claim text.