Process for production of insulin and insulin analogues
AssigneeBiogenomics Limited
InventorsArchana Krishnan, Sanjay Sonar, Damodar Thappa
Life of this patent
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Abstract
A process for production of insulin or insulin analogs by expression of Insulin or Insulin analogs through an expression vector in a host cell is provided. The expression vector includes a leader peptide of SEQ ID NO 3; a nucleotide sequence encoding an affinity tag linked to C-terminal end or N terminal end of nucleotide sequence of the leader peptide; and a nucleotide sequence encoding for a cleavage site ligated to nucleotide sequence of the leader peptide through nucleotide sequence encoding the affinity tag.
Claims16
A process for production of an insulin or insulin analogue, the process comprising expressing the insulin or insulin analogue through an expression vector in bacteria, wherein said expression vector comprises:
- a first nucleotide sequence encoding a leader peptide of SEQ ID NO:3;
- a second nucleotide sequence encoding an affinity tag, wherein the affinity tag is expressed linked to a C-terminal end or N-terminal end of said leader peptide;
- and
- a third nucleotide sequence encoding a cleavage site, wherein the third nucleotide sequence is ligated to the first nucleotide sequence encoding said leader peptide or to the second nucleotide sequence encoding said affinity tag.
A process for production of a insulin or insulin analogue, the process comprising expressing the insulin or insulin analogue through an expression vector in bacteria, wherein said expression vector comprises:
- a first nucleotide sequence encoding a leader peptide of SEQ ID NO:3;
- a second nucleotide sequence encoding an affinity tag, wherein the affinity tag is expressed linked to a C-terminal end or a N-terminal end of said leader peptide;
- a third nucleotide sequence, wherein the third nucleotide sequence encodes a cleavage site or is a Restriction Enzyme (RE) site, and wherein the third nucleotide sequence is ligated to the first nucleotide sequence of encoding said leader peptide or to the second nucleotide sequence encoding said affinity tag;
- a multiple cloning site (MCS) in upstream region of the first nucleotide sequence encoding said leader peptide;
- a fourth nucleotide sequence encoding a ribosome binding site (RBS);
- a promoter or operator sequence downstream of the fourth nucleotide sequence encoding said ribosome binding site (RBS);
- and
- a fifth nucleotide sequence encoding an antibiotic selection marker, wherein the fifth nucleotide sequence is upstream of said promotor or operator sequence.
A process for production of an insulin or insulin analogue, the process comprising expressing the insulin or insulin analogue through an expression vector in bacteria, wherein said expression vector comprises SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.